Part:BBa_J15202:Design

rbs+lacZ'

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Note the presence of a SacI site at the 5' end, overlapping the XbaI site; this is preserved when this biobrick is added to any other biobrick. The lacZ' gene was truncated by alteration of codon 77 from TGC to TGA (note: not TAATAA as recommended, due to an error; however, the part works fine).

Source

RBS synthetic; lacZ' from Escherichia coli BS, sequence derived from gi:146575.